کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866225 1470948 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly fluorescent carbon dots as selective and sensitive “on-off-on” probes for iron(III) ion and apoferritin detection and imaging in living cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Highly fluorescent carbon dots as selective and sensitive “on-off-on” probes for iron(III) ion and apoferritin detection and imaging in living cells
چکیده انگلیسی


• Highly luminescent N-doped carbon dots were prepared by an efficient one-step pyrolytic route.
• Sensitive and selective sensing of Fe3+ and apoferritin was achieved.
• The mechanism of the Fe3+ and apoferritin sensing was studied.
• The probe is applied to fluorescence imaging for cellular Fe3+ and apoferritin successfully.

Highly blue luminescent nitrogen-doped carbon dots (N-CDs) with a fluorescence quantum yield of 42.3% were prepared by an efficient one-step pyrolytic route from ethylenediaminetetraacetic acid and urea. The as-synthesized N-CDs were demonstrated as an effective fluorescent probe for label-free, selective and sensitive recognition of Fe3+ with a linear range of 0.5 μM to 2 mM and a detection limit of 13.6 nM due to Fe3+-quenched fluorescence (turn-off). The quenched fluorescence could be turned on after the addition of apoferritin owing to the removal of ferric species from the surface of N-CDs by apoferritin, making complex N-CDs/Fe3+ a selective apoferritin probe with a linear range of 0.1–25 μM and a detection limit as low as 2.6 nM. In addition, the application of this novel N-CDs-based probe for imaging Fe3+ ions and apoferritin in living cells suggest that this sensing system has great potential applications in biosensing, bioimaging, and many other fields.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 83, 15 September 2016, Pages 229–236
نویسندگان
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