کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866279 1470949 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel optical biosensor for direct and selective determination of serotonin in serum by Solid Surface-Room Temperature Phosphorescence
ترجمه فارسی عنوان
یک سنسور جدید بیوسنسور اپتیک برای تعیین مستقیم و انتخابی سروتونین در سرم به روش فشردگی درجه حرارت اتاق سطح
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• We have developed a novel optical sensor for analysing serotonin in complex matrices.
• It is free of pre-concentration and derivatization protocols.
• It is fast, simple, chip and the most environmental friendly method published so far.
• It is the first time that serotonin is analysed directly in serum with a non-enzymatic technique.

This paper describes a novel biosensor which combines the use of nanotechnology (non-woven nanofibre mat) with Solid Surface-Room Temperature Phosphorescence (SS-RTP) measurement for the determination of serotonin in human serum. The developed biosensor is simple and can be directly applied in serum; only requires a simple clean-up protocol. Therefore it is the first time that serotonin is analysed directly in serum with a non-enzymatic technique. This new approach is based on the covalent immobilization of serotonin directly from serum on a functional nanofibre material (Tiss®-Link) with a preactivated surface for direct covalent immobilization of primary and secondary amines, and the subsequent measurement of serotonin phosphorescent emission from the solid surface. The phosphorescent detection allows avoiding the interference from any fluorescence emission or scattering light from any molecule present in the serum sample which can be also immobilised on the nanofibre material. The determination of serotonin with this SS-RTP sensor overcomes some limitations, such as large interference from the matrix and high cost and complexity of many of the methods widely used for serotonin analysis.The potential applicability of the sensor in the clinical diagnosis was demonstrated by analysing serum samples from seven healthy volunteers. The method was validated with an external reference laboratory, obtaining a correlation coefficient of 0.997 which indicates excellent correlation between the two methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 82, 15 August 2016, Pages 217–223
نویسندگان
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