کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866340 1470959 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Parallel optical read-out of micromechanical pillars applied to prostate specific membrane antigen detection
ترجمه فارسی عنوان
خواندن موازی نوری از ستون های میکرومکانیکی برای تشخیص آنتی ژن غشای خاص پروستات استفاده می شود
کلمات کلیدی
سنسورهای میکرومکانیکی، تشخیص موازی نوری، آنتیژن غشایی خاص پروستات
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• High density micromechanical vertical sensors arrays are used for biomolecular detection.
• A novel approach to monitor the resonance frequency of tens of pillars in parallel is presented.
• Device sensitivity is improved over single pillar resonator by averaging signals coming from a multitude of sensors.
• Label free detection, faster adsorption kinetics, higher sensitivity, small analytes volumes are advantages coming from pillar technology.
• Prostate Specific Membrane Antigen is detected in the concentration range of clinical interest.

Micro and nanomechanical resonators represent a promising platform for proteins label-free detection because of their extreme sensitivity, fast response and low cost. Micro-pillars are columnar resonators that can be easily arranged in dense arrays of several thousand sensors in a squared mm. To exploit such a large density, however, a method for tracking independently micropillars resonance frequency is required. Here we present a detection method based on CCD imaging and software image analysis, which can measure the resonance frequency of tens of pillars in parallel. Acquiring simultaneously the frequency shift of up to 40 sensors and applying a proper statistical analysis, we were able to overcome the variability of the single measures improving the device sensitivity at low analyte concentration range.As a proof of concept, this method has been tested for the detection of a tumor marker, the Prostate Specific Membrane Antigen (PSMA). Pillars have been functionalized with an antibody against PSMA. The tumor marker (PSMA) has been detected in a range of concentrations between 300 pM and 100 nM, in buffer and in diluted bovine serum. The sensitivity of our method was limited only by the affinity constant of the antigen–antibody recognition. Moreover, this detection technique demonstrated to be effective in the 1–6 nM range, which is the window of PSMA concentration of clinical interest.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 72, 15 October 2015, Pages 393–399
نویسندگان
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