کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866364 | 1470966 | 2015 | 6 صفحه PDF | دانلود رایگان |
• Ligation chain reaction was applied to single-nucleotide polymorphism detection.
• Magnetic particles can not only simplify the operations but also efficiently reduce background.
• Chemiluminescence resonance energy transfer imaging was performed on the magnetic particles.
• This strategy achieved a detection limit of 0.86 fM mutant DNA and positive mutation detection with a wild-type to mutant ratio of 10,000:1.
A novel ligation chain reaction (LCR) methodology for single-nucleotide polymorphism (SNP) detection was developed based on luminol–H2O2–horseradish peroxidase (HRP)-mimicking DNAzyme–fluorescein chemiluminescence resonance energy transfer (CRET) imaging on magnetic particles. For LCR, four unique target-complement probes (X and X⁎, YG and Y⁎) for the amplification of K-ras (G12C) were designed by modifying G-quadruplex sequence at 3′-end of YG and fluorescein at 5′-end of Y⁎. After the LCR, the resulting products of XYG/X⁎Y⁎ with biotin-labeled X⁎ were captured onto streptavidin-coated magnetic particles (SA-MPs) via specific biotin-SA interaction, which stimulated the CRET reaction from hemin/G-quadruplex-catalyzed luminol–H2O2 CL system to fluorescein. By collecting signals by a cooled low-light CCD, a CRET imaging method was proposed for visual detection and quantitative analysis of SNP. As low as 0.86 fM mutant DNA was detected by this assay, and positive mutation detection was achieved with a wild-type to mutant ratio of 10,000:1. This high sensitivity and specificity could be attributed to not only the exponential amplification and excellent discrimination of LCR but also the employment of SA-MPs. SA-MPs ensured the feasibility of the proposed strategy, which also simplified the operations through magnetic separation and separated the reaction and detection procedures to improve sensitivity. The proposed LCR-CRET imaging strategy extends the application of signal amplification techniques to SNP detection, providing a promising platform for effective and high-throughput genetic diagnosis.
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Journal: Biosensors and Bioelectronics - Volume 65, 15 March 2015, Pages 139–144