کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866384 | 1470966 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Elevated levels of platelet-derived microparticles in blood is a potential biomarker for pathologies like acute myocardial infarction and stroke.
• We have designed an electrochemical biosensor to detect platelet-derived microparticles in a drop of blood towards screening and early detection of ‘high-risk’ individuals.
• Electrodes were fabricated with layers of graphene oxide and specific antibody targeted against platelet-derived microparticles.
• Results with blood from patients with acute myocardial infarction validated our biosensor as a specific, sensitive, label-free and cost-effective tool for rapid point-of-care detection.
We report here design of a graphene oxide-based electrochemical biosensor for detection of platelet-derived microparticles (PMPs), a major risk factor for arterial pro-thrombotic pathologies like acute myocardial infarction and stroke. Electrodes were fabricated with immobilized layers of graphene oxide and a specific antibody targeted against active conformation of integrin αIIbβ3 on PMP surface. Results showed progressive rise in impedance in Nyquist plots with increasing number of PMPs in analyte. The sensor was highly specific for PMPs and did not identify microparticles originating from other cells. Blood obtained from patients diagnosed with acute myocardial infarction exhibited significantly higher values of impedance, consistent with larger number of circulating PMPs in these patients, as compared to samples from healthy individuals, thus validating biosensor as a specific, sensitive, label-free and cost-effective tool for rapid point-of-care detection of PMPs at bedside. Our biosensor is most ideal for mass population screening programs at periphery-level healthcare units with limited resources. It is aimed at early detection of individuals having higher imminent cardiovascular risk, as well as for routine analysis, which in turn would contribute to better management and survival of screened ‘high-risk’ subjects.
Journal: Biosensors and Bioelectronics - Volume 65, 15 March 2015, Pages 274–280