کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866728 1470978 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The unfolding of G-quadruplexes and its adverse effect on DNA—gold nanoparticles-based sensing system
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The unfolding of G-quadruplexes and its adverse effect on DNA—gold nanoparticles-based sensing system
چکیده انگلیسی


• The adsorption of DNAs in G-quadruplex solutions onto gold nanoparticles was observed.
• The adsorption behavior was investigated in three widely used guanine-rich sequences.
• ICP-AES was used to discover the occurrence of the unfolding of G-quadruplexes.
• The potential unfolding by gold nanoparticles has adverse effect on the biosensing system.

The adsorption of DNAs in G-quadruplex solution onto 13 nm gold nanoparticles (AuNPs) was studied through monitoring of the localized surface plasmon resonance (LSPR) absorbance of 13 nm AuNPs at 520 and 650 nm (A650/A520) in the solutions of three widely studied guanine-rich sequences, TBA(5′-GGTTGGTGTGGTTGG-3′), PW17(5′-GGGTAGGGCGGGTTGGG-3′), and PSO (5′-GGGTTAGGGTTAGGGTTAGGG-3′). It was found that the degree of adsorption of DNAs in Pb2+ stabilized G-quadruplex (G-Pb2+) solutions is up to 93% after more than 5 h of incubation. Furthermore, the lead concentrations in the solutions containing G-quadruplex and AuNP were analyzed by an inductively coupled plasma atomic emission spectrometer. The results showed that Pb2+ had been released from the G-quadruplexes, which means the G-quadruplexes may be unfolded in the presence of AuNP. This interaction between G-quadruplexes and AuNP demonstrated that long time incubation between DNAs and AuNPs would possibly make it unable to distinguish G-quadruplex from ssDNA. Thus, a biosensing system consisting of PW17 and AuNPs was developed to detect Pb2+. It was found that the LSPR responses at A650/A520 were sensitive to [Pb2+]. However, the sensitivity of the system was interfered by the potential unfolding of PW17-Pb2+ in the presence of AuNPs. This unexpected adverse effect of AuNPs on DNA-based biosensors should be taken into consideration in the future development of biosensing systems that are based on ssDNA aptamers and unmodified AuNPs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 53, 15 March 2014, Pages 479–485
نویسندگان
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