کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866761 1470979 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sub-femtomole detection of 16s rRNA from Legionella pneumophila using surface plasmon resonance imaging
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sub-femtomole detection of 16s rRNA from Legionella pneumophila using surface plasmon resonance imaging
چکیده انگلیسی


• Surface plasmon resonance (SPR) and Quantum dots (QD) for Legionellosis detection.
• Sub-femtomole L. pneumophila 16s rRNA was detected using capture and detector probes.
• Optimal 16s rRNA fragmentation and QD signal amplification parameters were achieved.
• Detection of 1 pM 16s rRNA (0.45 femtomole) was achieved in less than 3 h.

Legionellosis has been and continues to be a life-threatening disease worldwide, even in developed countries. Given the severity and unpredictability of Legionellosis outbreaks, developing a rapid, highly specific, and sensitive detection method is thus of great pertinence. In this paper, we demonstrate that sub-femtomole levels of 16s rRNA from pathogenic Legionella pneumophila can be timely and effectively detected using an appropriate designed capture, detector probes, and a QD SPRi signal amplification strategy. To achieve specific and sensitive detection, optimal hybridization conditions and parameters were implemented. Among these parameters, fragmentation of the 16s rRNA and further signal amplification by QDs were found to be the main parameters contributing to signal enhancement. The appropriate design of the detector probes also increased the sensitivity of the detection system, mainly due to secondary structure of 16s rRNA. The use of 16s rRNA from L. pneumophila allowed for the detection of metabolically active pathogens with high sensitivity. Detection of 16s rRNA in solutions as diluted as 1 pM at 450 μL (0.45 femtomole) was achieved in less than 3 h, making our approach suitable for the direct, timely, and effective detection of L. pneumophila within man-made water systems.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 52, 15 February 2014, Pages 129–135
نویسندگان
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