کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
866829 1470988 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of lead (II) with a “turn-on” fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection of lead (II) with a “turn-on” fluorescent biosensor based on energy transfer from CdSe/ZnS quantum dots to graphene oxide
چکیده انگلیسی

Graphene oxide (GO) sheets are mixed with the aptamer-functionalized CdSe/ZnS quantum dots (QDs). Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QD ensemble, which enables the energy transfer from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer–QD ensemble assay acts as a “turn-on” fluorescent sensor for Pb2+ detection. When Pb2+ ions are present in the assay, the interaction of Pb2+ with the aptamer induces a conformational change in the aptamer, leading to the formation of a G-quadruplex/Pb2+ complex. As a result, the QDs that are linked to the G-quadruplex/Pb2+ complex are detached from the GO sheet, which “turns on” the fluorescence of the QDs. This sensor exhibits a limit of detection of 90 pM and excellent selectivity toward Pb2+ over a wide range of metal ions. The experiments have provided direct evidence that the fluorescence of QDs is quenched by GO via the nano-metal surface energy transfer (NSET) mechanism rather than the conventional Förster resonance energy transfer (FRET) process.

Figure optionsDownload as PowerPoint slideHighlights
► An ultra-sensitive fluorescent sensor has been developed for lead (II) detection.
► The fluorescence quenching of QDs is due to the nanometal surface energy transfer.
► This sensor exhibits a limit of detection of 90 pM and excellent selectivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 43, 15 May 2013, Pages 69–74
نویسندگان
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