کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866927 | 1470987 | 2013 | 4 صفحه PDF | دانلود رایگان |
Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R2 value of 0.990 and coefficient of variance ∼5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.
► Highly luminescent aqueous CdTe quantum dots synthesized.
► Quantum dots conjugated with specific antibody used as nanobioprobe.
► Dual fluorescence cum electrochemical format for HbA1c detection.
► Correlation coefficient of 96% for electrochemical detection with HPLC.
► Highly reproducible and promising HbA1c detection platform.
Journal: Biosensors and Bioelectronics - Volume 44, 15 June 2013, Pages 132–135