کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
866941 | 1470984 | 2013 | 6 صفحه PDF | دانلود رایگان |
► We report a highly sensitive detection of thrombin using a SERS-based magnetic aptasensor.
► Aptamer-conjugated gold nanoparticles were used as sensitive sensing probes.
► This overcomes the problem of the slow diffusion-limited kinetics on a solid substrate.
► The proposed method is a good clinical tool for the diagnosis of a thrombotic disease.
This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA15) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA29)-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27 pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease.
Journal: Biosensors and Bioelectronics - Volume 47, 15 September 2013, Pages 62–67