Simultaneous detection of biomolecular interactions and surface topography using photonic forcemicroscopy

We developed a photonic force microscope that can map multiple parameters simultaneously, including the surface topography and biomolecular interactions. To track the position of the probe bead and to determine contact position with the sample surface, we adopted a video analysis method using the diffraction pattern of monochromatic light passing through the probe bead. To demonstrate the capability of the microscope, we report the simultaneous measurement of the molecule distribution of DNA oligonucleotides on the surface, the binding strength of DNA hybridization between the bead and surface, and the topography of the smooth molded surface.

► We developed a photonic force microscope that can map multiple parameters simultaneously.
► We demonstrated simultaneous detection of the surface topography and biomolecular interactions.
► We expect that this technique can be applied to detect and map multiple parameters of soft matter and biomaterials.