کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867134 1470989 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An integrated sensing system for detection of cholesterol based on TiO2–graphene–Pt–Pd hybridnanocomposites
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An integrated sensing system for detection of cholesterol based on TiO2–graphene–Pt–Pd hybridnanocomposites
چکیده انگلیسی

In this article, we used the TiO2–graphene–Pt–Pd hybrid nanocomposites (TGPHs) as an enhanced element of the integrated sensing platform for increasing the surface area as well as improving the electronic transmission rate. Subsequently, Au nanoparticles (AuNPS) and cholesterol oxidase (ChOx) were successively self-assembled to TGPHs with high load amount and superior biological activity. The morphology of TGPHs and stepwise fabrication processes were characterized with cyclic voltammetry (CV), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Based on the efficiently catalytic ability of TGPHs and AuNPS, the fabricated biosensor exhibited wide linear ranges of responses to cholesterol in the concentration ranges of 5.0×10−8−5.9×10−4 M, the limit of detection was 0.017 μM (S/N=3). The response time was less than 7 s and the Michaelis–Menten constant (Kmapp))was found as 0.21 mM. The fabricated biosensor was further tested using real food samples egg, meat, margarine and fish oil, showing that the biosensor has the potential to be used as a facile cholesterol detection tool in food and supplement quality control.

Highlight
► Developed a multi-step chemical approach to synthesize TGPHs hybrid nanocomposites.
► TGPHs have good biocompatibility and can enlarge the electrode area.
► The fabricated biosensor was further tested using real food samples.
► The biosensor has the potential to be used as a facile cholesterol detectiontool.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 42, 15 April 2013, Pages 532–538
نویسندگان
, , , ,