کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867251 909779 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive and antifouling impedimetric aptasensor for the determination of thrombin in undiluted serum sample
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sensitive and antifouling impedimetric aptasensor for the determination of thrombin in undiluted serum sample
چکیده انگلیسی

A highly sensitive and attractive antifouling impedimetric aptasensor for the determination of thrombin in undiluted serum sample was developed. The aptasensor was fabricated by co-assembling thiol-modified anti-thrombin binding aptamer, dithiothreitol and mercaptohexanol on the surface of gold electrode. The performance of aptasensor was characterized by atomic force microscopy, contact angle and electrochemical impedance spectroscopy. In the measurement of thrombin, the change in interfacial electron transfer resistance of aptasensor was monitored using a redox couple of Fe(CN)63−/4−. The increase in the electron transfer resistance was linearly proportional to the concentration of thrombin in the range from 1.0 to 20 ng/mL and a detection limit of 0.3 ng/mL thrombin was achieved. The fabricated aptasensor displayed attractive antifouling properties and allowed direct quantification of extrinsic thrombin down to 0.08 ng/mL in undiluted serum sample. This work provides a promising strategy for clinical application with impressive sensitivity and antifouling characteristics.


► A simple, highly sensitive and attractive antifouling impedimetric aptasensor for determination of thrombin in undiluted serum sample was developed.
► A ternary monolayer was fabricated by co-assembling thiol-modified anti-thrombin binding aptamer, dithiothreitol and mercaptohexanol on gold electrode to form aptasensor.
► The fabricated aptasensor displayed attractive antifouling properties and it allowed the direct quantification of target thrombin down to 0.08 ng/mL in undiluted serum sample.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 39, Issue 1, 15 January 2013, Pages 324–328
نویسندگان
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