کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867252 909779 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hybridization assay of insect antifreezing protein gene by novel multilayered porous silicon nucleic acid biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Hybridization assay of insect antifreezing protein gene by novel multilayered porous silicon nucleic acid biosensor
چکیده انگلیسی

A fabrication of a novel simple porous silicon polybasic photonic crystal with symmetrical structure has been reported as a nucleic acid biosensor for detecting antifreeze protein gene in insects (Microdera puntipennis dzhungarica), which would be helpful in the development of some new transgenic plants with tolerance of freezing stress. Compared to various porous silicon-based photonic configurations, porous silicon polytype layered structure is quite easy to prepare and shows more stability; moreover, polybasic photonic crystals with symmetrical structure exhibit interesting optical properties with a sharp resonance in the reflectance spectrum, giving a higher Q factor which causes higher sensitivity for sensing performance. In this experiment, DNA oligonucleotides were immobilized into the porous silicon pores using a standard crosslink chemistry method. The porous silicon polybasic symmetrical structure sensor possesses high specificity in performing controlled experiments with non-complementary DNA. The detection limit was found to be 21.3 nM for DNA oligonucleotides. The fabricated multilayered porous silicon-based DNA biosensor has potential commercial applications in clinical chemistry for determination of an antifreeze protein gene or other genes.


► Porous silicon-based symmetrical structure as a DNA biosensor is proposed.
► High specificity in performing controlled experiments with non-complementary DNA.
► AFP gene from insects was first directly detected by a label-free biosensor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 39, Issue 1, 15 January 2013, Pages 329–333
نویسندگان
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