کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867256 909779 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensor
چکیده انگلیسی

A Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR) sensor was fabricated using spherical gold nanoparticles (Au NPs) on a flattened end-face of the optical fiber. The Au NPs were easily synthesized by the Turkevich method and were immobilized on the end-face of the optical fiber by using a self-assembled monolayer (SAM). In order to examine the possibility of its application as a biosensor for label-free immunoassays, the fabricated FO LSPR sensor was used for the detection of the antibody–antigen reaction of interferon-gamma (IFN-γ) and the limit of detection (LOD) was approximately 2 pg/ml. Herein, The antibodies and bovine serum albumins (BSAs) were immobilized on the Au NPs by physisorption. Also, the FO LSPR sensor was used for the detection of a prostate-specific antigen (PSA) and the LOD was 1 pg/ml below. The fabricated FO LSPR sensor can be used for real-time label-free immunoassay having fast detection time, high resolution and sensitivity. In addition, the proposed sensor platform has the advantages of low cost, simple optical setup, remote sensing, simple fabrication, real-time detection, low sample volume, and potential application to in-vivo detection systems.


► A biosensor was developed by using a Fiber-Optic Localized Surface Plasmon Resonance.
► The sensor can rapidly detect the antibody–antigen reaction of an interferon-gamma.
► It also can detect a prostate-specific antigen with high resolution and sensitivity.
► The biosensor can be applied for clinical testing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 39, Issue 1, 15 January 2013, Pages 346–351
نویسندگان
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