کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867272 | 909780 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Label-free electrochemical immunosensors based on surface-initiated atom radical polymerization Label-free electrochemical immunosensors based on surface-initiated atom radical polymerization](/preview/png/867272.png)
A novel label-free immunosensing strategy for sensitive detection of tumor necrosis factor-alpha antigen (TNF-α) via surface-initiated atom transfer radical polymerization (SI-ATRP) was proposed. In this strategy, the Au electrode was first modified by consecutive SI-ATRP of ferrocenylmethyl methacrylate (FMMA) and glycidyl methacrylate (GMA), and TNF-α antibody was coupled to the copolymer segment of GMA (PGMA) by aqueous carbodiimide coupling reaction. Subsequently, the target TNF-α antigen was captured onto the Au electrode surface through immunoreaction. The whole process was confirmed by scanning electron microscopy (SEM) and surface plasmon resonance (SPR) measurements. With introduction of redox polymer segment of FMMA (PFMMA) as electron-transfer mediator, the antigen-coupled Au electrode exhibited well electrochemical behavior, as revealed by cyclic voltammetry measurement. This provided a sensing platform for sensitive detection of TNF-α with a low detection limit of 3.9 pg mL−1. Furthermore, the “living” characteristics of the ATRP process can not only be readily controlled but also allow further surface functionalization of the electrodes, thus the proposed method presented a way for label-free and flexible detection of biomolecules.
Figure optionsDownload as PowerPoint slideHighlights
► The successive ATRP of FMMA and GMA endow co-fixing both signal and target molecules.
► The loading of ferrocene moieties was highly increased for each bio-recognition event.
► A detection limit of approximate 4 pg mL−1 was obtained due to the signal amplification.
► The signal molecules were directly existed in the polymer brushes simplified the experiment procedure.
► The SI-ATRP provided a versatile mean for fabrication of immunosensor with desirable sensitivity.
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 79–85