کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867298 909780 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Voltammetric investigation of DNA damage induced by nitrofurazone and short-lived nitro-radicals with the use of an electrochemical DNA biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Voltammetric investigation of DNA damage induced by nitrofurazone and short-lived nitro-radicals with the use of an electrochemical DNA biosensor
چکیده انگلیسی

Electrochemical behavior of nitrofurazone (NFZ) was investigated with the use of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The pH-dependence of NFZ was studied at a glassy carbon electrode (GCE) in ethanol/Britton–Robinson buffer (30:70), and short-lived nitro-radicals were generated by the reduction of NFZ at high pHs (>7.0). In the presence of DNA, the DPV peak current of NFZ decreased and the peak potential shifted negatively, which indicated that there was an electrostatic interaction between NFZ and DNA. An electrochemical dsDNA/GCE biosensor was prepared to study the DNA damage produced in the presence NFZ; this process was followed with the use of the Co(phen)32+ electroactive probe. Also, the oxidation peaks of guanosine (750 mV) and adenosine (980 mV) indicated that DNA damage was related directly to the nitro-radicals. Experiments demonstrated that DNA damage occurred via two different steps while NFZ was metabolized and nitro-radicals were produced. Novel work with AFM on the NFZ/DNA interaction supported the suggestion that in vivo, the nitro-radicals were more cytotoxic than the NFZ molecules. A linear DPV calibration plot was obtained for NFZ analysis at a modified dsDNA/GCE (concentration range: 2.50×10−6–3.75×10−5 mol L−1; limit of detection: 8.0×10−7 mol L−1), and NFZ was determined successfully in pharmaceutical samples.


► Electrochemical method was used to imitate the metabolic process of NFZ.
► Indirect and direct methods were used to detect the DNA damage by DNA biosensor.
► Quantitative determination of the NFZ by DNA biosensor.
► The interaction between nitro-radicals and DNA was intuitively studied.
► Both NFZ and its nitro-radicals can damage DNA.
► The metabolic product nitro-radicals were more dangerous than NFZ itself.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 245–251
نویسندگان
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