کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867300 909780 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction
چکیده انگلیسی

A protein-free, isothermal, self-amplified nucleic acid sensing system which was a G-quadruplex integrated hybridization chain reaction (GQ-HCR) system was developed. The G-quadruplex was closed two-thirds in the loop and one-third in the stem of one of the GQ-HCR hairpin probes. In the absence of the target molecule, the GQ-HCR probes stayed as inactive meta-stable hairpin structures and the G-quadruplex was inert. Reversely, the GQ-HCR probes could be cross-opened to start a hybridization chain reaction and the closed G-quadruplex could be released to be free when the GQ-HCR probes came across the target molecule. The GQ-HCR nucleic acid sensing system could detect as low as 7.5 nM ssDNA or RNA by the colorimetric method and 4 nM ssDNA by the fluorometric method. Less than 10 copies of dsDNA template could also be detected when PCR was combined with the GQ-HCR system (PCR+GQ-HCR). Because of these advantages, the GQ-HCR system was also studied for application in visual chip detection to obtain a satisfactory repeatable and specific result.


► Protein-free, isothermal, self-amplified nucleic acid sensing system GQ-HCR via the integration of intact G-Quadruplex and HCR for the colorimetric or fluorimetric detection of single-stranded DNA or RNA.
► In order to detect double stranded DNA and get much better detection sensitivity, we improved the detection system further through the combination of PCR with GQ-HCR.
► GQ-HCR system is an ideal platform to develop visual-chip based nucleic acid detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 258–263
نویسندگان
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