کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
867311 | 909780 | 2012 | 6 صفحه PDF | دانلود رایگان |
A universal label-free metal ion sensor design strategy was developed on the basis of a metal ion-specific DNA/RNA-cleaving DNAzyme and a G-quadruplex DNAzyme. In this strategy, the substrate strand of the DNA/RNA-cleaving DNAzyme was designed as an intramolecular stem–loop structure, and a G-rich sequence was caged in the double-stranded stem and could not form catalytically active G-quadruplex DNAzyme. The metal ion-triggered cleavage of the substrate strand could result in the release of the G-rich sequence and subsequent formation of a catalytic G-quadruplex DNAzyme. The self-blocking mechanism of the G-quadruplex DNAzyme provided the sensing system with a low background signal. The signal amplifications of both the DNA/RNA-cleaving DNAzyme and the G-quadruplex DNAzyme provided the sensing system with a high level of sensitivity. This sensor design strategy can be used for metal ions with reported specific DNA/RNA-cleaving DNAzymes and extended for metal ions with unique properties. As examples, dual DNAzymes-based Cu2+, Pb2+ and Hg2+ sensors were designed. These “turn-on” colorimetric sensors can simply detect Cu2+, Pb2+ and Hg2+ with high levels of sensitivity and selectivity, with detection limits of 4 nM, 14 nM and 4 nM, respectively.
► A dual DNAzymes-based metal ion sensor design strategy was developed.
► Signal amplifications by DNA/RNA-cleaving and G-quadruplex DNAzymes provide high sensitivity.
► The self-blocking mechanism of G-quadruplex DNAzyme provides low background signal.
► As examples, highly sensitive and selective Cu2+, Pb2+ and Hg2+ sensors were designed.
► This sensor design strategy can also be easily used for other metal ions.
Journal: Biosensors and Bioelectronics - Volume 38, Issue 1, October–December 2012, Pages 331–336