Anti-epithelial cell adhesion molecule monoclonal antibody conjugated fluorescent nanoparticle biosensor for sensitive detection of colon cancer cells

► A nanosensor for detecting colon cancer cells is reported. ► The covalent immobilization using NaIO4 oxidation method is more effective than using Glutaraldehyde method. ► The fluorescence microscopy imaging and flow cytometer (FCM) experiments demonstrate the sensitivity and selectivity of nanosensor. ► The membrane and nuclear staining show the distribution and abundance of EpCAM in cells' membrane.