کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867474 909783 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microelectrode array biosensor for studying carbohydrate-mediated interactions
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Microelectrode array biosensor for studying carbohydrate-mediated interactions
چکیده انگلیسی

Carbohydrate-mediated host-pathogen interactions are essential to bacterial and viral pathogenesis, and represent an attractive target for the development of antiadhesives to prevent infection. We present a versatile microelectrode array-based platform to investigate carbohydrate-mediated protein and bacterial binding, with the objective of developing a generalizable method for screening inhibitors of host-microbe interactions. Microelectrode arrays are well suited for interrogating biological binding events, including proteins and whole-cells, and are amenable to electrochemical derivitization, facilitating rapid deposition of biomolecules. In this study, we achieve microelectrode functionalization with carbohydrates via controlled polymerization of pyrrole to individual microelectrodes, followed by physisorption of neoglycoconjugates to the polypyrrole-coated electrodes. Bioactivity of the immobilized carbohydrates was confirmed with carbohydrate-binding proteins (lectins) detected by both fluorescent and electrochemical means. The platform's ability to analyze whole-cell binding was demonstrated using strains of Escherichia coli and Salmonella enterica, and the dose-dependent inhibition of S. enterica by a soluble carbohydrate antiadhesive.


► Functionalization of a microelectrode biosensor with carbohydrates via polypyrrole.
► Confirm bioactivity using fluorescence and electrochemical detection.
► Array is suitable for studying whole-cell bacterial interactions.
► Demonstrate dose-dependent inhibition of Salmonella enterica.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 34, Issue 1, 15 April 2012, Pages 253–260
نویسندگان
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