کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867540 909785 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay
چکیده انگلیسی

Fluorescence resonance energy transfer (FRET) between two quantum dots of different sizes causes fluorescence quenching. Hereby a binding site pre-blocking approach is proposed to avoid this effect. Pre-binding of glucose on the donor occupies the binding sites and thus blocks resonance energy transfer between the two quantum dots, protecting the fluorescence from being quenched. A glucose assay is developed based on this approach. The glucose content is correlated with the fluorescence difference in the absence and in the presence of glucose. In practice, Green QDs–Con A conjugates are used as donors and Red QDs–NH2-glu conjugates as acceptors to form FRET system. The inhibition of fluorescence quenching is then measured in the presence of glucose. A linear calibration graph is achieved within 0.1–2.0 mmol L−1, along with a detection limit of 0.03 mmol L−1 and a RSD of 2.1% (1.0 mmol L−1). 91–105% of glucose in serum and urine samples is recovered. It is worth mentioning that the present glucose assay approach also generates a fluorescence chromatic difference imaging, and the color display clearly identifies the glucose contents by visual detection with a distinguishing ability of ca. 0.5 mmol L−1. The present approach can potentially be used for the clinical determination of glucose in biological samples which can be further developed into a glucose sensor.


► FRET between two quantum dots causes fluorescence quenching. A binding site pre-blocking assay approach for avoiding this phenomenon is proposed.
► Pre-binding of glucose on the donor occupies the binding sites and blocks resonance energy transfer between quantum dots, protecting the fluorescence from being quenched.
► A glucose assay approach is developed based on this approach.
► This system generates a fluorescence chromatic difference image, which can identify glucose contents by visual detection with a distinguishing ability of ca. 0.5 mM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 32, Issue 1, 15 February 2012, Pages 82–88
نویسندگان
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