کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
867736 909791 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ferrocenemonocarboxylic–HRP@Pt nanoparticles labeled RCA for multiple amplification of electro-immunosensing
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ferrocenemonocarboxylic–HRP@Pt nanoparticles labeled RCA for multiple amplification of electro-immunosensing
چکیده انگلیسی

A multiple amplification immunoassay was proposed to detect alpha-fetoprotein (AFP), which was based on ferrocenemonocarboxylic–HRP conjugated on Pt nanoparticles as labels for rolling circle amplification (RCA). Firstly, the capture antibody (anti-AFP) was immobilized on glass carbon electrode (GCE) deposited nano-sized gold particles. After a typical immuno-sandwich protocol, primary DNA was immobilized by labeling secondary antibody, which acted as a precursor to initiate RCA. The products of RCA provide large amount of sites to link detection DNAs, which were labeled by signal probes (ferrocenemonocarboxylic) and horseradish peroxidase (HRP). Moreover, the enzymatic amplification signals could be produced by the catalysis of HRP and Pt nanoparticles with the addition of H2O2. These lead to multiple amplification signals monitoring by electrochemical instrument and further resulted in high sensitivity of the immunoassay with the detection limit of 1.7 pg/mL.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 11, 15 July 2011, Pages 4601–4604
نویسندگان
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