کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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867881 | 909795 | 2010 | 4 صفحه PDF | دانلود رایگان |
Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalisation of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen™) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 7, 15 March 2010, Pages 1816–1819