کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868034 909801 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free biosensing by means of periodic lattices of high aspect ratio SU-8 nano-pillars
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Label-free biosensing by means of periodic lattices of high aspect ratio SU-8 nano-pillars
چکیده انگلیسی

We developed biophotonic sensing arrays of 60 × 60 μm2 made of periodic lattices of high aspect ratio SU-8 nano-pillars in order to demonstrate their capability for label-free molecule detection, as well as the sensitivity enhancement in comparison with a single layer of SU-8. The biophotonic sensing arrays, that we call BICELLs (Biophotonic sensing cells), are interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR). We monitored the surface immobilization of bovine serum albumin (BSA) antigen and anti-BSA antibody (aBSA) recognition. The bioassay exhibits a limit of detection (LOD) in the order of 2 ng/ml limited by the wavenumber uncertainty during the interrogation process. We also estimated and compared the theoretical biolayer thickness with previous results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 12, 15 August 2010, Pages 2553–2558
نویسندگان
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