کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868189 909803 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A miniature chip for protein detection based on magnetic relaxation switches
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A miniature chip for protein detection based on magnetic relaxation switches
چکیده انگلیسی

A miniature multi-sample chip for protein detection with a bench-top magnetic resonance imager was created on the basis of magnetic relaxation switches. The chip was assessed with two protein systems. Both qualitative and quantitative results for the target proteins were obtained by image analysis and relaxation time measurement, respectively. The detection of prostate specific antigen, the serum marker of human prostate cancer, showed a linear concentration range of 17.3–43.2 ng mL−1 and a detection limit of 13.7 ng mL−1. As proof of concept, the analysis of 18 samples with a volume of 6.37 μL each was completed in 26 min by this chip. This technique may become an easy and efficient approach for rapid and high-throughput protein assay and protein–protein interaction screening.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 26, Issue 5, 15 January 2011, Pages 2258–2263
نویسندگان
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