کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
868375 | 909807 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A stable and selective electrochemical biosensor for the liver enzyme alanine aminotransferase (ALT) A stable and selective electrochemical biosensor for the liver enzyme alanine aminotransferase (ALT)](/preview/png/868375.png)
An electrochemical method to determine alanine aminotransferase (ALT) activity over its normal and elevated physiological range was developed based upon detection of l-glutamate at a glutamate oxidase-modified platinum electrode. Measurements were carried out in the presence of ALT co-substrates l-alanine and α-ketoglutarate and current response from either the oxidation of hydrogen peroxide or the re-oxidation of the mediator ferrocene carboxylic acid was employed. The enzyme electrode was tested over a 6-month period and found to retain 79% of its original activity towards ALT detection with >200 measurements performed over this time. Signals associated with interfering electroactive species (ascorbic acid and uric acid) were eliminated using background subtraction at a denatured glutamate oxidase enzyme electrode. The sensitivity of the device was found to be 0.845 nA U−1 L ALT with t90 = 180 s, linear range 10–1000 U L−1 and LOD of 3.29 U L−1 using amperometry at Eapp = 0.4 V vs. Ag/AgCl at 308 K (35 °C).
Journal: Biosensors and Bioelectronics - Volume 24, Issue 9, 15 May 2009, Pages 2926–2930