کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868714 909812 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1
چکیده انگلیسی

A simple, rapid and highly sensitive piezoelectric immunosensor has been proposed and applied to detect aflatoxin B1 (AFB1). It is unlikely that direct binding of small molecules such like AFB1 to the piezoelectric sensor surface could result in a satisfactory detection limit and sensitivity. Thus, indirect competitive immunoassay technique had been used for the detection of the target and gold nanoparticles (GNP) been employed as a ‘weight label’ to the secondary antibody for amplifying the response. This method is proven in its ability to detect AFB1 down to a level of 0.01 ng mL−1 in artificially contaminated milk, which is comparable to or even exceeding the sensitivity of microtitre plate ELISA. Furthermore, the frequency responses of the immunoassay are linearly correlated to the logarithm of AFB1 concentration in the range of 0.1–100 ng mL−1. The sensor could be regenerated under very mild conditions simply by immersing the sensor into glycine buffer solution to desorb the combined antibody. It is found that the as-renewed sensor could be reused at least 9 runs without obvious loss of sensing sensitivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 8, 15 April 2009, Pages 2580–2585
نویسندگان
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