کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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868823 | 909814 | 2010 | 4 صفحه PDF | دانلود رایگان |
In this work, the Z-domain of protein A was expressed as a recombinant-fusion protein on the outer membrane of Escherichia coli by using “Autodisplay” technology, and the outer membrane with Z-domains was layered on a SPR biosensor as a highly sensitive molecular recognition layer. The outer membrane layer formation was demonstrated by using various surfaces with controlled hydrophobicity. The effect of orientation control by the outer membrane with Z-domains was estimated to have far higher sensitivity and far improved limit of detection (LOD) in comparison to the conventional immunoassay by using a model analyte, horseradish peroxidase (HRP). The SPR biosensor with the outer membrane layer was applied to the detection of C-reactive protein (CRP). From these experiments, the LOD of SPR biosensor was estimated to improve more than 100-fold compared to the SPR biosensor with the antibody-layer by physical adsorption.
Journal: Biosensors and Bioelectronics - Volume 25, Issue 5, 15 January 2010, Pages 1225–1228