کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
868979 909817 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly sensitive electrochemical detection of human telomerase activity based on bio-barcode method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Highly sensitive electrochemical detection of human telomerase activity based on bio-barcode method
چکیده انگلیسی

In the present study, an electrochemical method for highly sensitive detection of human telomerase activity was developed based on bio-barcode amplification assay. Telomerase was extracted from HeLa cells, then the extract was mixed with telomerase substrate (TS) primer to perform extension reaction. The extension product was hybridized with the capture DNA immobilized on the Au electrode and then reacted with the signal DNA on Au nanoparticles to form a sandwich hybridization mode. Electrochemical signals were generated by chronocoulometric interrogation of [Ru(NH3)6]3+ that quantitatively binds to the DNA on Au nanoparticles via electrostatic interaction. This method can detect the telomerase activity from as little as 10 cultured cancer cells without the polymerase chain reaction (PCR) amplification of telomerase extension product.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 25, Issue 11, 15 July 2010, Pages 2543–2547
نویسندگان
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