کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869105 909821 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA
چکیده انگلیسی

A new multidetection biosensor has been developed using the electrochemical properties of cylinder-shaped conducting polypyrrole grown on miniaturized graphite electrodes. Our objective was to conceive a sensitive, labelless and real-time DNA sensor for biomedical diagnosis. In a first step, copolymers bearing both ferrocene redox markers and oligonucleotide probes were selectively electro-addressed on microchip electrodes. Then, the study of their voltammetric response upon the addition of DNA targets revealed that the hybridization was efficiently transduced through the variation of ferrocene oxidation intensity. Using this technique, a good selectivity between Human Immunodeficiency Virus and Hepatitis B Virus targets was obtained. It was indeed possible to directly follow the hybridization. Complementary DNA detection limit reached 100 pM (3 fmol in 30 μL), which represents a good performance for such a practical, labelless and real-time sensor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 23, Issue 5, 15 December 2007, Pages 735–740
نویسندگان
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