کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869221 909825 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optical sensing of biomedically important polyionic drugs using nano-sized gold particles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Optical sensing of biomedically important polyionic drugs using nano-sized gold particles
چکیده انگلیسی

A simple optical method for the sensing of biomedically important polyionic drugs, protamine and heparin based on the reversible aggregation and de-aggregation of gold nanoparticles (AuNPs) is described. The polycationic protamine induces the aggregation of negatively charged citrate-stabilized AuNPs, resulting in a shift in the surface plasmon (SP) band and a consequent color change of the AuNPs from red to blue. Addition of polyanionic heparin dissipates the aggregated AuNPs due to its strong affinity to protamine and the blue color changes to the native color. The color change was monitored using UV–vis spectrophotometry. The aggregation and de-aggregation was confirmed by transmission electron microscopic (TEM) measurements. The degree of aggregation and de-aggregation is proportional to the concentration of added protamine and heparin, allowing their quantitative detection. The change in the absorbance and SP band position has been used to monitor the concentration of protamine and heparin. This optical method can quantify protamine and heparin as low as 0.1 μg/ml and 0.6 μg/ml, respectively and the calibration is linear for a wide range of concentration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 23, Issue 8, 14 March 2008, Pages 1285–1290
نویسندگان
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