کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
869387 | 909829 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Quantitative oligonucleotide microarray data analysis with an artificial standard probe strategy Quantitative oligonucleotide microarray data analysis with an artificial standard probe strategy](/preview/png/869387.png)
Quantitative data analysis is an important element in several applications of DNA microarray, including mRNA expression profiling and estimation of infectious doses for pathogens. Here, we introduce an artificial standard probe strategy for quantitative pathogen detection using an oligonucleotide chip as a model system. The standard capture probe sequence was artificially designed to prevent non-specific hybridization with bacterial targets. Based on the fluorescence intensities of artificial standard spots, the raw fluorescence intensity data for specific spots could be corrected to generate linear correlations with target concentrations. Therefore, our novel artificial standard probe may be effectively applied for the correction of chip-to-chip variations and quantitative data analysis of a one-color labeled DNA microarray system.
Journal: Biosensors and Bioelectronics - Volume 23, Issue 11, 15 June 2008, Pages 1738–1744