کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
869469 | 909830 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Highly sensitive lactate biosensor by engineering chitosan/PVI-Os/CNT/LOD network nanocomposite Highly sensitive lactate biosensor by engineering chitosan/PVI-Os/CNT/LOD network nanocomposite](/preview/png/869469.png)
A novel chitosan/PVI-Os(polyvinylimidazole-Os)/CNT(carbon nanotube)/LOD (lactate oxidase) network nanocomposite was constructed on gold electrode for detection of lactate. The composite was nanoengineered by selected matched material components and optimized composition ratio to produce a superior lactate sensor. Positively charged chitosan and PVI-Os were used as the matrix and the mediator to immobilize the negatively charged LOD and to enhance the electron transfer, respectively. CNTs were introduced as the essential component in the composite for the network nanostructure. FESEM (field emission scan electron microscopy) and electrochemical characterization demonstrated that CNT behaved as a cross-linker to network PVI and chitosan due to its nanoscaled and negative charged nature. This significantly improved the conductivity, stability and electroactivity for detection of lactate. The standard deviation of the sensor without CNT in the composite was greatly reduced from 19.6 to 4.9% by addition of CNTs. With optimized conditions the sensitivity and detection limit of the lactate sensor was 19.7 μA mM−1 cm−2 and 5 μM, respectively. The sensitivity was remarkably improved in comparison to the newly reported values of 0.15–3.85 μA mM−1 cm−2. This novel nanoengineering approach for selecting matched components to form a network nanostructure could be extended to other enzyme biosensors, and to have broad potential applications in diagnostics, life science and food analysis.
Journal: Biosensors and Bioelectronics - Volume 22, Issue 12, 15 June 2007, Pages 3288–3292