کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869476 909831 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Formaldehyde assay by capacitance versus voltage and impedance measurements using bi-layer bio-recognition membrane
چکیده انگلیسی

A novel formaldehyde sensitive biosensor based on bacterial formaldehyde dehydrogenase (FDH) as a bio-recognition element has been developed. The bio-recognition membrane had bi-layer architecture and consisted of FDH, cross-linked with albumin, and of the cofactor NAD at a high concentration level (first layer). The second layer was a negatively charged Nafion membrane, which prevented a leakage of negatively charged NAD molecules from the bio-membrane. As transducers, gold electrodes SiO2/Si/SiO2/Ti/Au and electrolyte-insulator-semiconductor Si/SiO2 (EIS) structures have been used. Changes in capacitance and impedance properties of the bio-recognition membrane have been used for monitoring formaldehyde concentration in a bulk solution. It has been shown that formaldehyde can be detected within a concentration range from 1 μM to 20 mM depending on the type of transduction used, with a detection limit of 1 and 100 μM for gold-based and EIS-based transducers, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issue 5, 15 December 2006, Pages 575–581
نویسندگان
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