کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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869616 | 909834 | 2007 | 7 صفحه PDF | دانلود رایگان |
The presence of an estrogen binding protein (EBP) and an endogenous ligand in three yeast species was first reported in 1982/1983. The ligand was shown to be 17β-estradiol and the binding affinities of EBP were demonstrated to be similar to those of rat estrogen receptors. This report describes detection of the behaviour of a putative estrogen binding protein in Saccharomyces cerevisiae using a double mediator electrochemical detection system. The response to estrogen is shown to be quantitative with signals detectable from 10−8 to 10−14 M. An incubation period of 5 h is established and a method to block electrochemical signals produced by the catabolism of exogenous substrates is demonstrated to be effective. The system provides a method that permits the use of wild type S. cerevisiae to quantify estrogens.
Journal: Biosensors and Bioelectronics - Volume 22, Issue 11, 15 May 2007, Pages 2493–2499