کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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869636 | 909834 | 2007 | 7 صفحه PDF | دانلود رایگان |
We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10−7 to 10−3 M. The lower detection limit of approximately 10−7 M for paraoxon was an order of magnitude better than the OPH-based potentiometric and optical biosensors based on pH modulation. There was a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard errors of 5% and 15%, respectively. OPs measured using this technique included parathion and diisopropyl fluorophosphate (DFP) in the order of paraoxon > DFP > parathion. The conformational change of the OPH was most likely the main origin of MCL bending.
Journal: Biosensors and Bioelectronics - Volume 22, Issue 11, 15 May 2007, Pages 2636–2642