کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
869655 | 909834 | 2007 | 5 صفحه PDF | دانلود رایگان |
This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NADP+. The product, NADPH, initiates the irreversible the hydroxylation of p-hydroxybenzoate by HBH in the presence of oxygen to produce 3,4-dihydroxybenzoate, which results in a detectable signal due to its oxidation at the working electrode. The sensor shows a broad linear detection range between 2 μM and 1000 μM with a low detection limit of 1.2 μM. Also, it has a fast measuring time which can achieve 95% of the maximum current response in 20 s after the addition of a given concentration of glucose-6-phosphate with a short recovery time (2 min).
Journal: Biosensors and Bioelectronics - Volume 22, Issue 11, 15 May 2007, Pages 2754–2758