کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
869757 909838 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel enzyme biosensor for hydrogen peroxide via supramolecular associations
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel enzyme biosensor for hydrogen peroxide via supramolecular associations
چکیده انگلیسی

A polythiolated-β-cyclodextrin polymer was synthesized and used as a coating material for gold electrodes. The functionalized electrodes were employed for immobilizing adamantane-modified horseradish peroxidase via supramolecular associations. The enzyme-containing electrode was used as an amperometric biosensor device with 1 mM hydroquinone as electrochemical mediator. The biosensor exhibited a fast amperometric response (10 s), a good linear response toward H2O2 concentrations between 28 μM and 5.5 mM, and a low detection limit of 7 μM. The biosensor showed a sensitivity of 109 μA/M cm2 and retained 98% of its initial electrocatalytic activity after 40 days of storage at 4 °C in 50 mM sodium phosphate buffer pH 7.0. The host–guest supramolecular nature of the immobilization method was confirmed by cyclic voltammetry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 24, Issue 7, 15 March 2009, Pages 2028–2033
نویسندگان
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