کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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869837 | 909841 | 2006 | 7 صفحه PDF | دانلود رایگان |
A flow-through PQC/DNA biosensor system is developed by combining sequential flow polymerase chain reaction (PCR) products denaturing prior to piezoelectric quartz crystal (PQC) detection via hybridization of ssDNA. The PQC/DNA biosensor is fabricated based on complex formation of neutravidin/biotinylated probe in 0.2 M NaCl in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.5). Results show that the coating fabricated provides a desirable quality with satisfactory performance. Its application for Escherichia coli detection under controlled flow at 0.02 mL/min for denaturing PCR products and 10 mL/min for transferring solution between reactors and delivering samples to detector to reduce rehybridization leads to significant improvement in repeatability (R.S.D. < 6%, n = 5) and sensitivity (ΔF = 34 Hz/1000 E. coli cells) as compared to existing manual method (R.S.D. = 19%, n = 5 and ΔF = 26 Hz/1000 E. coli cells, respectively). Down to 23 E. coli cells are detected, satisfying the HKEPD requirements for E. coli count in beach water.
Journal: Biosensors and Bioelectronics - Volume 22, Issue 4, 15 October 2006, Pages 506–512