کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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869937 | 909842 | 2008 | 5 صفحه PDF | دانلود رایگان |

In this paper, a novel method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium–tin-oxide (ITO) and used as a template to form cellular micropatterns. A suspension of C2C12 cells was introduced into the patterning device between the upper slide and the bottom IDA. In the present system, the n-DEP force is induced by applying an ac voltage (typically 12 Vpp, 1 MHz) to direct cells toward a weaker region of electric field strength. The cells aligned above one of the bands of IDA within 1 min since the aligned areas on the slide were regions with the lower electric field. The application of an ac voltage for 5 min allows the cells to adsorb onto the cell culture slide. After removing excess cells, the second cell type was patterned in lines using the same method as with the first set of cells. Periodic and alternate cell lines incorporating two cell types were also fabricated by changing the ac voltage mode. A second cell type was introduced into the device and guided to other areas to form a different pattern. The described system enables two cell types to be patterned in 15 min. The patterning method provides a novel tool for use in fundamentals studies of cell biology based on cell–cell interactions between different cell types.
Journal: Biosensors and Bioelectronics - Volume 24, Issue 4, 1 December 2008, Pages 1043–1047