کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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870099 | 909849 | 2008 | 4 صفحه PDF | دانلود رایگان |
Staphylococcus aureus (S. aureus)-specific bacteriophage was used as a probe for detection of methicillin-resistant S. aureus (MRSA) in aqueous solution using a novel optical method. Biorecognition phage monolayers transferred to glass substrates using Langmuir–Blodgett (LB) technique were exposed individually to MRSA in solution at logarithmic concentrations ranging from 106 to 109 cfu/ml, and observed for real-time binding using a CytoViva™ optical light microscope system. Results indicate that LB monolayers possessed high levels of elasticity (K), measuring 22 and 29 mN/m for 109 and 1011 pfu/ml phage concentrations, respectively. Near-instantaneous MRSA–phage binding produced 33 ± 5%, 10 ± 1%, 1.1 ± 0.1%, and 0.09 ± 0.01% coverage of the substrate that directly correlated to a decrease in MRSA concentrations of 109, 108, 107, and 106 cfu/ml. The exclusive selectivity of phage monolayers was verified with Salmonella enterica subsp. enterica serovar typhimurium (S. typhimurium) and Bacillus subtilis.
Journal: Biosensors and Bioelectronics - Volume 24, Issue 1, 15 September 2008, Pages 151–154