کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870472 909869 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control
چکیده انگلیسی

Being beneficial from dramatic progress in bio-chemical and micro-electro-mechanical technologies, DNA manipulation devices can be miniaturized to expedite amplification processing for virus detection. Polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) are two typical examples of them. In this report, a micro-RT-PCR (μRT-PCR) chip is designed to quantitatively detect tumor viruses. Test sample reservoirs, RT-PCR meanders, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control module is well developed by embedding signal amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high efficacy for heat transfer, exemption of fluid flow clogging and superior sensitivity to precisely control the required reaction temperatures in all heated zones of μRT-PCR unit. Intensive simulations and experiments are presented to verify the validity of the fabricated bio-chip.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issue 2, 15 August 2006, Pages 313–317
نویسندگان
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