Nano-immunoassay with improved performance for detection of cancer biomarkers

Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders signal to be more stable against photo-induced damage and carbonaceous contamination.From the Clinical EditorThis study discusses strategies to improve the performance of nano-immunoassays utilizing surface-enhanced Raman scattering (SERS) effect , an analytical technique with high sensitivity that holds a great promise for early cancer biomarker detection.

An improved performance of the surface-enhanced Raman scattering (SERS) based nano-immunoassay for detection of cancer biomarkers is reported. Optimization of the immunoassay components, capture substrate and ERL, resulted in improved reproducibility of the measured SERS readout signal. Stability and sensitivity of the assay are further improved by using a thin protective layer applied on top of the assay.Figure optionsDownload high-quality image (149 K)Download as PowerPoint slide