Nanostructured and thermoresponsive recombinant biopolymer-based microcapsules for the delivery of active molecules

Multilayer capsules conceived at the nano- and microscales are receiving increasing interest due to their potential role as carriers of biomolecules for drug delivery and tissue engineering. Herein we report the construction of microcapsules by the sequential adsorption of chitosan and a biomimetic elastin-like recombinamer into nanostructured layers on inorganic microparticle templates. The release profile of bovine serum albumin, which was studied at 25 and 37 °C, shows higher retention and Fickian diffusion at physiological temperature. The self-assembled multilayers act as a barrier and allowed for sustained release over 14 days. The capsules studied are non-cytotoxic towards L929 cells, thereby suggesting multiple applications in the fields of biotechnology and bioengineering, where high control of the delivery of therapeutics and growth/differentiation factors is required.From the Clinical EditorIn this paper, the construction of microcapsules by sequential adsorption of chitosan and a biomimetic, elastin-like recombinamer into nanostructured layers on inorganic microparticle templates is reported. The layers demonstrated sustained drug release over 14 days. These microcapsules are non-cytotoxic toward L929 cells, suggesting multiple applications where high control of drug or growth factor delivery is required.

Graphical AbstractStimuli-responsive microcapsules were produced using layer-by-layer through the sequential adsorption of chitosan and a biomimetic elastin-like recombinamer into nanostructured layers and onto inorganic microparticle templates. We present such system as a non-cytotoxic biomolecule reservoir with tunable permeability by simply varying the number of layers and the temperature at a physiological relevant range.Figure optionsDownload high-quality image (111 K)Download as PowerPoint slide