Micropatterned stretching system for the investigation of mechanical tension on neural stem cells behavior

In this study, we developed a feasible and reliable stretching platform combined with photolithography and microfluidic techniques to investigate the effect of directional tensile force and guiding microchannel on neural stem cell (NSC) behavior. Different stretching modes and culture conditions were conducted to investigate the mechanoresponse of NSCs on micropatterned substrate and to verify the effects of tension on NSCs maturation, axon sprouting, neurite outgrowth and orientation. From the results, we found that neurite extension and axon elongation were significantly enhanced and neurites were more directional orientated to parallel direction as stretching was experienced. The mechanical tension apparently influenced NSCs differentiation toward neuronal cells under stretching condition. The neuronal maturity also showed a significant difference when compared with parallel and vertical micropatterned channels. It is suggested that mechanical tension not only can guide neurites orientation and direction, but also promote their elongation length and trigger neural stem cells differentiation into mature neuronal cells.From the Clinical EditorThis group of investigators report the development of a feasible and reliable stretching platform combined with photolithography and microfluidic techniques to investigate the effects of directional tensile force and guiding microchannel on neural stem cell behavior. They demonstrate that neurite extension and axon elongation could be significantly enhanced, and neuronal maturity can also be improved.

Graphical AbstractThe schematic of micropatterned PDMS substrate and the linear stage stretching system. The OM and SEM pictures show the aligned microchannels. The immunocytochemistry results illustrate the specific neuronal protein markers obviously shown in parallel and perpendicular microchannels after applied tensile force.Figure optionsDownload high-quality image (99 K)Download as PowerPoint slide