کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8838876 | 1613217 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GDNF family receptor α-1 in the catfish: Possible implication to brain dopaminergic activity
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کلمات کلیدی
3β-Hydroxysteroid dehydrogenasesiRNAsGDNFMPTPHPLC-ECDMedulla oblongataTphTBSDPHORFTeleostsCYP19A1Hsd3bGFRα-1Rapid amplification cDNA endsPBSPEIl-3,4-dihydroxyphenylalanine - L-3،4-دی هیدروکسی فنیل آلانینl-DOPA - L-DOPAPCA - PCAsmall interfering RNAs - RNA های تداخل کوچکaromatase - آروماتازamino acid - آمینو اسیدEstradiol-17β - اتراستیل 17 بتاPerchloric acid - اسید پرکلریکThalamus - تالاموسTris-buffered saline - تریس بافر شورtyrosine hydroxylase - تیروزین هیدروکسیلازCont - حسابRET - حقRoom temperature - دمای اتاقDopaminergic - دوپامینرژیکdigoxigenin - دیگوکسین ژنBrain development - رشد مغز days post hatch - روزه گرفتنFemale - زنDIG - شماPituitary - غدهٔ زیرمغزی یا هیپوفیزGlial cell line-derived neurotrophic factor - فاکتور نوروتروفی مشتق از سلول گلیاییPhosphate buffered saline - فسفات بافر شورopen reading frame - قاب خواندن بازRace - مسابقهMale - نرnorepinephrine - نوراپی نفرینtryptophan hydroxylase - هیدروکسیلاز تریپتوفانPolyethylenimine - پلی اتیلنcatecholamines - کاتکول امینHigh performance liquid chromatography-electrochemical detection - کروماتوگرافی مایع با عملکرد بالا - تشخیص الکتروشیمیاییControl - کنترل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
Glial cell line-derived neurotrophic factor (GDNF)is a potent trophic factor that preferentially binds to GDNF family receptor α-1 (GFRα-1)by regulating dopaminergic (DA-ergic) neuronsin brain. Present study aimed to evaluate the significance of GFRα-1 expression during early brain development in catfish. Initially, the full-length cDNA of GFRα-1 was cloned from adult brain which showed high homology with other vertebrate counterparts. Quantitative PCR analysis of tissue distribution revealed ubiquitous expression of GFRα-1 in the tissues analyzed with high levels in female brain and ovary. Significant high expression was evident in brain at 75 and 100â¯days post hatch females than the respective age-match males. Expression of GFRα-1 was high in brain during the spawning phase when compared to other reproductive phases. Localization of GFRα-1 revealed its presence in preoptic area-hypothalamus which correlated well with the expression profile in discrete areas of brain in adult catfish. Transient silencing of GFRα-1through siRNA lowered expression levels of GFRα-1, which further down regulated the expression of certain brain-specific genes. Expression of GFRα-1 in brain declined significantly upon treatment with the 1-methyl-1,2,3,6-tetrahydropyridinecausing neurodegeneration which further correlated with catecholamines (CA), L-3,4-dihydroxyphenylalanine, DA and norepinephrine levels. Taken together, GFRα-1 plausibly entrains gonadotropin-releasing hormone and gonadotropin axiseither directly or indirectly, at least by partially targeting CA-ergic activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 140, June 2018, Pages 270-280
Journal: Brain Research Bulletin - Volume 140, June 2018, Pages 270-280
نویسندگان
Sajwan-Khatri Mamta, Balasubramanian Senthilkumaran,