کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840534 | 1614689 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Impact of Perineuronal Net Digestion Using Chondroitinase ABC on the Intrinsic Physiology of Cortical Neurons
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کلمات کلیدی
ECMPBSsACSFPFAPMBSFRMPresting membrane potentialsWFAPNNs - PNN هاInhibitory interneurons - اینترنورونهای مهاریfast spiking - سریع اسپایکینگperineuronal nets - شبکه های perineuronalAHP - فرایند تحلیل سلسلهمراتبیBarrel cortex - قشر بشکهExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریWisteria floribunda agglutinin - ویستریا فلوریبند آگلوتیینینparaformaldehyde - پارافرمالدهیدaction potential - پتانسیل عمل after-hyperpolarization - پس از hyperpolarization
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perineuronal nets (PNNs) are a form of aggregate Extracellular Matrix (ECM) in the brain. Recent evidence suggests that the postnatal deposition of PNNs may play an active role in regulating neuroplasticity and, potentially, neurological disorders. Observations of high levels of PNN expression around somas, proximal dendrites, and axon initial segments of a subtype of neurons have also led to proposals that PNNs may modulate the intrinsic properties of the neurons they ensheathe. While high levels of PNNs are postnatally expressed throughout the neocortex, it is still unclear how they impact the neuronal physiology of the many classes and subtypes of neurons that exist. In this study, we demonstrate that Chondroitinase ABC digestion of PNNs from acute cortical slices from juvenile mice (P28-35) resulted in neuron-specific impacts on intrinsic physiology. Fast spiking (FS) interneurons showed decreased input resistance, resting membrane potential (RMP), reduced action potential (AP) peaks and altered spontaneous synaptic inputs. Low-Threshold Spiking interneurons showed altered rebound depolarizations and decreased frequency of spontaneous synaptic inputs. Putative excitatory neurons; regular spiking, bursting, and doublet phenotypes did not demonstrate any alterations. Our data indicate that chABC-sensitive PNNs may specifically regulate the intrinsic and synaptic physiology of inhibitory interneurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 388, 15 September 2018, Pages 23-35
Journal: Neuroscience - Volume 388, 15 September 2018, Pages 23-35
نویسندگان
Philip Chu, Reena Abraham, Kumarie Budhu, Usma Khan, Natalia De Marco Garcia, Joshua C. Brumberg,