کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840699 | 1614694 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased Expression of Transcription Factor SRY-box-Containing Gene 11 (Sox11) Enhances Neurite Growth by Regulating Neurotrophic Factor Responsiveness
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کلمات کلیدی
GFPGap43ATF3GDNFNGFDRGIRESSDSdorsal root ganglion - گانگلیون ریشه پشتیRegeneration - باززاییphosphate buffer - بافر فسفاتNeurite growth - رشد نوریتMolecular biology - زیستشناسی مولکولیinternal ribosomal entry site - سایت ورودی ریبوزوم داخلیsodium dodecyl sulfate - سدیم دودسیل سولفاتTranscription factor - عامل رونویسیNeurotrophic factor - عامل نوروترفیکnerve growth factor - فاکتور رشد عصبGlial cell line-derived neurotrophic factor - فاکتور نوروتروفی مشتق از سلول گلیاییactivating transcription factor 3 - فعال کردن عامل رونویسی 3polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازgreen fluorescent protein - پروتئین فلورسنت سبزgrowth-associated protein 43 - پروتئین مرتبط با رشد 43dorsal root ganglia - گانگلیس ریشه پشتی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The peripherally projecting axons of dorsal root ganglion (DRG) neurons readily regenerate after damage while their centrally projecting branches do not regenerate to the same degree after injury. One important reason for this inconsistency is the lack of pro-regeneration gene expression that occurs in DRG neurons after central injury relative to peripheral damage. The transcription factor SRY-box-containing gene 11 (Sox11) may be a crucial player in the regenerative capacity of axons as previous evidence has shown that it is highly upregulated after peripheral axon damage but not after central injury. Studies have also shown that overexpression or inhibition of Sox11 after peripheral nerve damage can promote or block axon regeneration, respectively. To further understand the mechanisms of how Sox11 regulates axon growth, we artificially overexpressed Sox11 in DRG neurons in vitro to determine if increased levels of this transcription factor could enhance neurite growth. We found that Sox11 overexpression significantly enhanced neurite branching in vitro, and specifically induced the expression of glial cell line-derived neurotrophic factor (GDNF) family receptors, GFRα1 and GFRα3. The upregulation of these receptors by Sox11 overproduction altered the neurite growth patterns of DRG neurons alone and in response to growth factors GDNF and artemin; ligands for GFRα1 and GFRα3, respectively. These data support the role of Sox11 to promote neurite growth by altering responsiveness of neurotrophic factors and may provide mechanistic insight as to why peripheral axons of sensory neurons readily regenerate after injury, but the central projections do not have an extensive regenerative capacity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 382, 1 July 2018, Pages 93-104
Journal: Neuroscience - Volume 382, 1 July 2018, Pages 93-104
نویسندگان
Michael P. Jankowski, Lauren Miller, H. Richard Koerber,