کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841012 | 1614704 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Superficial Dorsal Horn Neurons from “Tamamaki” Mice and Stability of their GAD67-EGFP Phenotype in Defined-Medium Organotypic Culture
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کلمات کلیدی
aCSFDulbecco's modified Eagle’s mediumlamina IIIEIACDsEPSCsNGFHyperpolarization-activated cyclic nucleotide-gatedGBSSOTCDMEMFBSeGFPROIHCNBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز acid citrate dextrose - اسید سیترات دکستروزElectrophysiology - الکتروفیزیولوژیsynaptic transmission - انتقال سیناپسیNeuropathic pain - درد نوروپاتیکfetal bovine serum - سرم جنین گاوColony-stimulating factor - فاکتور تحریک کننده Colonynerve growth factor - فاکتور رشد عصبBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزorganotypic cultures - فرهنگ های ارگانوپاتیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیRegions Of Interest - مناطق مورد علاقهGenetically modified mice - موش های اصلاح شده ژنتیکیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Defined medium organotypic cultures (DMOTC) containing spinal dorsal horn neurons are especially useful in studying the etiology and pharmacology of chronic pain. We made whole-cell recordings from neurons in acutely isolated mouse spinal cord slices or from those maintained in DMOTC for up to 6â¯weeks. In acute slices, neurons in the substantia gelatinosa exhibited 7 different firing patterns in response to 800-ms depolarizing current commands; delay (irregular), delay (tonic), tonic, regular firing, phasic, initial bursting and single spiking. Initial bursting and regular firing neurons are not found in rat substantia gelatinosa. In acute slices from “Tamamaki” mice that express enhanced green fluorescent protein (EGFP) under the control of the glutamic acid decarboxylase 67 (GAD67) promotor, tonic, phasic and regular firing neurons exhibited the strongest GABAergic (GAD67-EGFP+) phenotype. Delay (tonic) and delay (irregular) neurons almost never expressed GAD67 (GAD67-EGFPâ) and are likely glutamatergic. All seven phenotypes were preserved in mouse spinal cord neurons in DMOTC prepared from e12 embryos and the GAD67-EGFP+ phenotype continued to associate with phasic and regular firing neurons. Only 3 out of 51 GAD67-EGFP+ neurons exhibited a delay (tonic) firing pattern. Modifications to the mouse genome thus continue to be expressed when embryonic neurons develop in vitro in DMOTC. However, analysis of the amplitude and interevent interval of spontaneous EPSCs (sEPSCs) indicated substantial re-arrangement of synaptic connections within the cultures. Despite this, the characteristics and age-dependence of asynchronous oscillatory activity, as monitored by multiphoton Ca2+ imaging, were similar in acute slices and in DMOTC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 372, 21 February 2018, Pages 126-140
Journal: Neuroscience - Volume 372, 21 February 2018, Pages 126-140
نویسندگان
Paul A. Boakye, Emma K.A. Schmidt, Vladimir Rancic, Bradley Kerr, Klaus Ballanyi, Peter A. Smith,